Horseradish peroxidase as a tracer for capillary permeability studies.
نویسندگان
چکیده
منابع مشابه
The Permeability of Dragonfly Malpighian Tubule Cells to Protein Using Horseradish Peroxidase as a Tracer
Theoretical models developed to explain the coupled solute-water transport in various absorptive epithelia consider the movement of water to be a passive process produced by the localized secretion of ions into channels associated with the epithelium (cf. 6, 9-13, 20, 28) . This standing gradient hypothesis has been extended to Malpighian tubules of insects (7, 14) . In these particular cells, ...
متن کاملThe Ultrastructural Basis of Alveolar-capillary Membrane Permeability to Peroxidase Used as a Tracer
The permeability of the alveolar-capillary membrane to a small molecular weight protein, horseradish peroxidase (HRP), was investigated by means of ultrastructural cytochemistry. Mice were injected intravenously with HRP and sacrificed at varying intervals. Experiments with intranasally instilled HRP were also carried out. The tissue was fixed in formaldehyde-glutaraldehyde fixative. Frozen sec...
متن کاملThe Ultrastructural Basis of Capillary Permeability Studied with Peroxidase as a Tracer
The transendothelial passage of horseradish peroxidase, injected intravenously into mice, was studied at the ultrastructural level in capillaries of cardiac and skeletal muscle. Peroxidase appeared to permeate endothelial intercellular clefts and cell junctions. Abnormal peroxidase-induced vascular leakage was excluded. Neutral lanthanum tracer gave similar results. The endothelial cell junctio...
متن کاملStudies on Horseradish Peroxidase
The kinetics of the oxidation of p-cresol by Compound II of horseradish peroxidase has been studied by the stopped flow technique at an ionic strength of 0.11 from pH 2 to 11. In acid solution the reaction is kinetically first order in jcresol, but in the alkaline region a saturation effect attributable to complex formation is observed. At very high pH an additional second order reaction betwee...
متن کاملThe permeability of muscle capillaries to horseradish peroxidase
The main objective of this study was to determine the pathways by which horseradish peroxidase (HRP) can cross the endothelium of muscle capillaries. Specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg HRP, at 4 min after intervenous injection of varied amounts of HRP, and at 4 min after intervenous injections in various ...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.10.512320